Gel Documentation Systems
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UVItec chemiluminescence documentation systems
are designed with the exacting demands of the technique in mind, using the
highest quality components to give the very best in image quality and
sensitivity. This range also takes into account the potential application
cross-over between chemiluminescent and fluorescent techniques by offering
the option of a system with both capabilities (UVIprochemi) as well as one
dedicated to chemiluminescence (UVIchemi).
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The
sensitivity and image quality of the chemiluminescence system is assured
by the exceptionally low noise of the 12-bit cooled camera. The camera
cooling efficiency is maximized by housing it in a separate compartment
with its own cooling device. Because it is a dedicated system, the darkroom
cabinet can be made as small as possible to eliminate light leakage and maximize
sensitivity. The sample tray can be moved up or down to vary the field of
view and the superb quality fixed focal length lens allows razor sharp focusing
in all sample positions.
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Features:
Highest
Quality CCD camera
The CCD camera used in
UVIchemi has a resolution of 1.3 million pixels which gives a clear and
sharp image while the high quantum efficiency of 70% at 450nm ensures
unrivalled sensitivity. The baseline noise level of the camera, which
limits the sensitivity and dynamic range, is extremely low at less than
1% of the 12-bit scale of 4096 intensity levels. Noise remains low even
at exposure times of more than one hour, giving the system extraordinary
ultimate sensitivity. The camera noise is limited by efficient and direct
on-chip cooling of the CCD sensor to -55°C below ambient by a two-stage
forced-air Peltier device. A second Peltier actually cools the camera
compartment to combat high ambient temperatures that would otherwise
affect system performance. "Binning" mode, in which four CCD
pixels are combined into one, can be used to increase sensitivity even
further, but at the expense of image resolution.
Innovative Darkroom Cabinet
The UVIchemi darkroom
cabinet is small and compact, which maximizes sensitivity by minimizing
camera-sample distance and also saves bench space. The top camera
compartment incorporates its own Peltier-cooling device, which keeps the
compartment at <20°C regardless of the outside temperature. The lower
sample compartment has six vertical positions for the sample tray
allowing variation of the field of view and the distance from the camera.
Simple manual adjustment of the high quality lens allows razor sharp focusing
at any sample position. Gentle overhead LED illumination enables sample
positioning without excessive glare. The doors to both compartments are
sealed with highly efficient latches.
Simple and Convenient Software Interface
UVIchemi software
incorporates the same characteristics of simplicity and convenience as
all other UVItec software packages. A convenient, user-friendly layout,
special acquisition toolbar and icon driven functions make use of the
software less cumbersome and more intuitive. Special automatic functions
are included to facilitate optimization of exposure times. The
"Automatic Exposure" function performs short test exposures in
"binning" mode and then calculates the optimum time for a full
resolution exposure. The "Test Signal" function performs a
series of binning exposures at user defined intervals and informs the
user when the chemiluminescence signal is sufficiently developed for
image acquisition. Other acquisition functions available include
"live" image display - a high frequency video display useful
for sample positioning and focusing; manual exposure where the user
defines the exposure time before initiating acquisition; video mode,
where a series of full resolution images of user defined exposure times
and intervals are acquired. The acquired images can then be saved as TIFF
files in either the full 12-bit or displayed 8-bit form.
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Designed
for a combination of chemiluminescence and high sensitivity fluorescence
documentation, UVIprochemi has many of the features of UVIchemi including
the same 12-bit, cooled camera and similar software interface. It differs
in the inclusion of a darkroom cabinet incorporating a UV
transilluminator plus overhead (epi)- UV and white lighting. There is a
single position sample tray for chemiluminescence documentation and a motorized
zoom lens ensures that fluorescent gels and chemiluminescent blots of
many different sizes may be optimally imaged. The software interface includes
all UVIchemi features plus the zoom lens controls in which aperture and
zoom are adjusted by selecting from pre-set values, and focus is adjusted
via two pairs of easy to use + and - buttons for coarse and fine
adjustment.
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System Specifications
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Model
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UVIchemi
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UVIprochemi
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Camera Specifications
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Resolution
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1.3 million pixels
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1.3 million pixels
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Output
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12-bit (4096 grey
levels)
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12-bit (4096 grey
levels)
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Dynamic range
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>4 orders of
magnitude
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>4 orders of
magnitude
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Quantum efficiency
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70% at 450nm
Direct, on-chip cooling of sensor to -55°C below ambient by two stage
Peltier device
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70% at 450nm
Direct, on-chip cooling of sensor to -55°C below ambient by two stage
Peltier device
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Darkroom cabinet
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DBT 6000 including:
Separate sample and Peltier-cooled camera compartments,
4 x LED lights for sample illumination
6-position sample tray
Overall exterior dimensions 680 x 350 x 350 (hxwxd), mm
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DBT 8000 including:
Separate sample and Peltier-cooled camera compartments,
Two position filter holder
Overhead (epi)-UV/white light illumination
Pull-out tray for mini UV transilluminator Single position
chemiluminescence sample tray Exterior control panel for all lighting
options. Overall exterior dimensions 920 x 540 x 430 (hxwxd), mm
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Lens
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High quality, fixed focal length,
17 x 0.95
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F1.4, 11.5 - 69 (6X) motorized zoom lens, independent software driven control of aperture, zoom
and focus
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Software interface
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PC based, linked to camera by PCI card interface, compatible with Windows® 98, 2000, XP
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PC based, linked to camera by PCI card interface, compatible with Windows® 98, 2000, XP
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Order information:
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Cat. No.
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Description
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286-001
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UVIchemi
chemiluminescence documentation system CAS6000 consisting of:
High quality, 1.3 megapixel, 12-bit cooled CCD camera, darkroom cabinet
DBT 6000, acquisition board and software, 17mm/F0.95 fixed focal length
lens, cables and user manual.
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286-002
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UVIprochemi
chemiluminescence/fluorescence documentation system CAS8000 consisting
of:
High quality, 1.3 megapixel, 12-bit cooled CCD camera, darkroom cabinet
DBT 8000 with epi-UV and white light illumination and filter holder,
narrow band-pass filter, mini transilluminator BTS 26M, acquisition board
and software, F1.4 11.5-69 (6X) motorized zoom lens, cables and user
manual
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